UBE2D1/2/3/4 Polyclonal antibody

UBE2D1/2/3/4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11677-1-AP

Synonyms

E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D1, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, human brain tissue, human heart tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

11677-1-AP targets UBE2D1/2/3/4 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag2278
Full Name ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003395
Gene symbol UBE2D3
Gene ID (NCBI) 7323
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Protocols

Product Specific Protocols
WB protocol for UBE2D1/2/3/4 antibody 11677-1-APDownload protocol
IHC protocol for UBE2D1/2/3/4 antibody 11677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Asian Pac J Cancer Prev

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation.

Authors - Ganiou Assani
humanIHC

Oncol Lett

UBE2D3 is a positive prognostic factor and is negatively correlated with hTERT expression in esophageal cancer.

Authors - Ge Ge Guan
humanIHC

Front Oncol

UBE2D3 Activates SHP-2 Ubiquitination to Promote Glycolysis and Proliferation of Glioma via Regulating STAT3 Signaling Pathway.

Authors - Zhenjiang Pan
humanWB

Oncotarget

UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.

Authors - Xiaojia Gao
humanWB

Sci Signal

Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.

Authors - Weinert Brian T BT