|Positive WB detected in||HEK-293 cells, HeLa cells, human brain tissue, human heart tissue, Jurkat cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
11677-1-AP targets UBE2D1/2/3/4 in WB, IHC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||UBE2D1/2/3/4 fusion protein Ag2278|
|Full Name||ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC003395|
|Gene ID (NCBI)||7323|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.
Asian Pac J Cancer Prev
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UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.
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