UNC79 Recombinant antibody

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  IHC staining of mouse brain using 84642-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84642-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 84642-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UNC79 antibody (84642-1-RR, Clone: 241628A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84642-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84642-1-RR against Human UNC79 were performed. The affinity constant is 11 nM.

UNC79 Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84642-1-RR

Clone No.241628A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Protein unc-79 homolog, KIAA1409, 241628A7

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84642-1-RR targets UNC79 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UNC79 fusion protein Ag35440
• Full Name: KIAA1409
• Calculated Molecular Weight: 295 kDa
• GenBank Accession Number: NM_020818.4
• Gene Symbol: UNC79
• Gene ID (NCBI): 57578
• RRID: AB_3672101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9P2D8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC79 is one of the key auxiliary subunits of the NALCN channel complex. Together with NALCN, FAM155A, and UNC80, it constitutes the NALCN channel body and is essential for maintaining neuronal excitability, motor function, pain sensitivity, and circadian rhythm (PMID: 35387979).