VAMP2 Monoclonal antibody

VAMP2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1G7E8

Cat no : 67822-1-Ig

Synonyms

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2



Tested Applications

Positive WB detected inrabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67822-1-Ig targets VAMP2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen VAMP2 fusion protein Ag17908
Full Name vesicle-associated membrane protein 2 (synaptobrevin 2)
Calculated molecular weight 13 kDa
Observed molecular weight19 kDa
GenBank accession numberBC002737
Gene symbol VAMP2
Gene ID (NCBI) 6844
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Protocols

Product Specific Protocols
WB protocol for VAMP2 antibody 67822-1-IgDownload protocol
IHC protocol for VAMP2 antibody 67822-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols