CoraLite® Plus 488-conjugated VAMP2 Monoclonal antibody

VAMP2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7E8

Cat no : CL488-67822

Synonyms

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Validation Data Gallery

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  IF Staining of U-87 MG using CL488-67822

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CoraLite® Plus 488 VAMP2 antibody (CL488-67822, Clone: 1G7E8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: U-87 MG cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67822 targets VAMP2 in IF applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: VAMP2 fusion protein Ag17908
• Full Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC002737
• Gene Symbol: VAMP2
• Gene ID (NCBI): 6844
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).