VEGFA Polyclonal antibody

• Featured Product
• KD/KO Validated

VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

484

Reviews (1)

Cat no : 19003-1-AP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Validation Data Gallery

• 

  WB analysis using 19003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19003-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of NIH/3T3 using 19003-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 9 publications below
• WB: See 367 publications below
• IHC: See 139 publications below
• IF: See 43 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

19003-1-AP targets VEGFA in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, goat, monkey, pig, rabbit, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag13500
• Full Name: vascular endothelial growth factor A
• Calculated molecular weight: 412 aa, 46 kDa
• Observed molecular weight: 45 kDa, 23 kDa
• GenBank accession number: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Publications

Species	Application	Title
mouse	WB,IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu ; KD Validated
mouse	WB	Light Sci Appl; Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model.; Authors - Lechan Tao
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi
human	WB	Bone Res; Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation; Authors - Zhuang Cui
human	IF	Nat Commun; A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery.; Authors - Kewei Ren
mouse	WB,IF	Nat Commun; Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.; Authors - Bin Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fiona (Verified Customer) (07-16-2019)

Good quality antibody and good value for money. Would purchase again.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

VEGFA Antibody Western Blot, validation (1:500 dilution) in ARPE-19 (Cat no:19003-1-AP)

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