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VEGF Antibody 145 Publications

Rabbit Polyclonal| Catalog number: 19003-1-AP

Featured Product KD/KO validated

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Con: 55 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, CoIP, ELISA
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Images See all

VEGF Antibody WB MCF-7 cells 19003-1-AP

MCF-7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

VEGF Antibody IHC human lung cancer tissue 19003-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

VEGF Antibody IHC human lung cancer tissue 19003-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, pig, rat, sheep, swine
Positive WB detected in:
MCF-7 cells, HeLa cells, SGC-7901 cells, U-937 cells
Positive IHC detected in:
human lung cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
VEGF fusion protein Ag13500
Full name:
vascular endothelial growth factor A
Calculated molecular weight:
412aa,46 kDa
Observed molecular weight:
25-30 kDa, 40-46 kDa
GenBank accession number:
BC065522
Gene ID (NCBI):
7422
Gene symbol
VEGFA
Synonyms
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

Background

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.


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