|Positive WB detected in||Jurkat cells, MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19003-1-AP targets VEGFA in WB, RIP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||goat, human, monkey, mouse, pig, rabbit, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VEGFA fusion protein Ag13500|
|Full Name||vascular endothelial growth factor A|
|Calculated molecular weight||412 aa, 46 kDa|
|Observed molecular weight||45 kDa, 25 kDa|
|GenBank accession number||BC065522|
|Gene ID (NCBI)||7422|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fiona (Verified Customer) (07-16-2019)
Good quality antibody and good value for money. Would purchase again.