Mouse VPS13C Polyclonal antibody, PBS Only

• 

  WB analysis using 28676-1-AP (same clone as 28676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 28676-1-AP (same clone as 28676-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28676-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28676-1-AP (same clone as 28676-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 28676-1-AP (same clone as 28676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28676-1-AP (VPS13C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28676-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VPS13C Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28676-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

BLTP5C, Intermembrane lipid transfer protein VPS13C, Kiaa3021, PARK23, Vacuolar protein sorting-associated protein 13C

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Product Information

28676-1-PBS targets VPS13C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29434

  Product name: Recombinant mouse VPS13C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3575-3699 aa of NM_177184

  Sequence: GIQRAAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDLLENHIKKLEGEAYQFHCAVPGNKRAVLMITNRRALFIKEVEILGHMSVDWQCLFEDFVCPPEVSENLLKISVKEQGLFHKKDSAN

• Full Name: vacuolar protein sorting 13C (yeast)
• Observed Molecular Weight: 422 kDa
• GenBank Accession Number: NM_177184
• Gene Symbol: Vps13c
• Gene ID (NCBI): 320528
• RRID: AB_2881191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8BX70
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).