VPS13C Polyclonal antibody

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  WB analysis using 28676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28676-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 28676-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28676-1-AP (VPS13C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

VPS13C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28676-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

BLTP5C, PARK23

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28676-1-AP targets VPS13C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29434

  Product name: Recombinant mouse VPS13C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3580-3705 aa of NM_177184

  Sequence: GIQRAAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDLLENHIKKLEGETYRYHCAIPGSKKTILMVTNRRVLCIKEVEILGLMCVDWQCPFEDFVFPPSVSENVLKISVKEQGLFHKKDSANQ

• Full Name: vacuolar protein sorting 13 homolog C (S. cerevisiae)
• Observed Molecular Weight: 422 kDa
• GenBank Accession Number: NM_001018088
• Gene Symbol: VPS13C
• Gene ID (NCBI): 54832
• RRID: AB_2881191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q709C8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Science; LYVAC/PDZD8 is a lysosomal vacuolator; Authors - Haoxiang Yang
mouse	WB	J Cell Biol; ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling.; Authors - William Hancock-Cerutti
human,mouse	WB,IHC,IF	iScience; Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1; Authors - Xiangyu Tan ; KD Validated
human		J Cell Biol; VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons; Authors - Leonie F Schrӧder
human	WB	Biomedicines; A Pilot Study on the Proteomics Profile of Serum Exosome-Enriched Extracellular Vesicles from Normal versus Individuals with Obesity-Related Insulin Resistance; Authors - Viswanathan Saraswathi
human	WB	Contact (Thousand Oaks); VPS13A and VPS13C Influence Lipid Droplet Abundance; Authors - Shuliang Chen ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (01-25-2021)

HEK293T lysates (10ug) ran on 4-12% gradient gel prior to transfer to nitrocellulose membrane. Membrane incubated overnight at 4 degrees with antibody (1:250). Goat anti-rabbit HRP secondary antibody (1:10,000) incubated with membrane for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

VPS13C Antibody Western Blot validation (1:250 dilution) in HEK293T (Cat no:28676-1-AP)