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VPS4A Polyclonal antibody

VPS4A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14272-1-AP

Synonyms

hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A



Tested Applications

Positive WB detected inU-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14272-1-AP targets VPS4A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitycanine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS4A fusion protein Ag5592
Full Name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Calculated molecular weight 49 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC047932
Gene symbol VPS4A
Gene ID (NCBI) 27183
RRIDAB_2878039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VPS4A antibody 14272-1-APDownload protocol
IHC protocol for VPS4A antibody 14272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Parasitol Res

Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum.

Authors - Francesca De Falco