Tested Applications
| Positive WB detected in | U-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue, rat stomach tissue, mouse pancreas tissue, rat kidney tissue, human stomach cancer tissue, rat lung tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14272-1-AP targets VPS4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5592 Product name: Recombinant human VPS4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 86-437 aa of BC047932 Sequence: KENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES Predict reactive species |
| Full Name | vacuolar protein sorting 4 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 48-55 kDa |
| GenBank Accession Number | BC047932 |
| Gene Symbol | VPS4A |
| Gene ID (NCBI) | 27183 |
| RRID | AB_2878039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UN37 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS4A antibody 14272-1-AP | Download protocol |
| IHC protocol for VPS4A antibody 14272-1-AP | Download protocol |
| WB protocol for VPS4A antibody 14272-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS4A antibody (Cat# 14272-1-AP) has 13 citations spanning journals including Nature, Nat Commun, Mol Cell, PNAS, Theranostics, Cell Rep, Autophagy, Life Sci, Cells, J Pharmacol Exp Ther, Biochem Biophys Res Commun, and Parasitol Res. Research fields cover autophagy (lipophagy, microautophagy, chaperone-mediated), ESCRT-III biology, STING signaling, HIV-1, CAR-T therapy, colorectal cancer, liver fibrosis, atherosclerosis, pyroptosis, and parasitic infection.
| Species | Application | Title |
|---|---|---|
Nat Commun Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy. | ||
Theranostics Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy | ||
Proc Natl Acad Sci U S A Negative interplay between HIV-1 Gag and amyloid precursor protein centers around competition for VPS4A and TSG101 | ||
Proc Natl Acad Sci U S A Endosome transcriptomics reveal trafficking of Cajal bodies into multivesicular bodies. | ||
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer used it for Western Blot at a 1:1000 dilution on mouse brain lysate and reported that it successfully detects endogenous VPS4A at a molecular weight of around 50 kD. Overall, the feedback confirms reliable performance and correct molecular weight detection in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nuo (Verified Customer) (11-07-2024) | This antibody can detect endogenous VPS4A with a MW of around 50 kD.
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