VPS4A/B Polyclonal antibody, PBS Only

• 

  WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 17673-1-AP (same clone as 17673-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

  IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 17673-1-AP (same clone as 17673-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Cat No. 17673-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1

---

Product Information

17673-1-PBS targets VPS4A/B in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11610

  Product name: Recombinant human VPS4B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-444 aa of BC039574

  Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

• Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 444 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC039574
• Gene Symbol: VPS4B
• Gene ID (NCBI): 9525
• RRID: AB_2215504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75351
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.