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  • KD/KO Validated

VPS4A/B Polyclonal antibody

VPS4A/B Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 17673-1-AP

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B



Tested Applications

Positive WB detected inY79 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, mouse skin tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17673-1-AP targets VPS4A/B in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS4A/B fusion protein Ag11610
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC039574
Gene symbol VPS4B
Gene ID (NCBI) 9525
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

Protocols

Product Specific Protocols
WB protocol for VPS4A/B antibody 17673-1-APDownload protocol
IF protocol for VPS4A/B antibody 17673-1-APDownload protocol
IP protocol for VPS4A/B antibody 17673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.

Authors - Haiqiong Wang
  • KD Validated
humanWB

J Extracell Vesicles

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Authors - Wang Jiang
ratWB,IF

EMBO J

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

Authors - Benjamin M Schwenk
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei