VPS4A/B Polyclonal antibody

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• VPS4A/B Polyclonal antibody (17673-1-AP)

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• KD/KO Validated

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 17673-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC (Intra), IP, ELISA

Synonyms

VPS4B, Cell migration-inducing gene 1 protein, EC:3.6.4.6, MIG1, Protein SKD1

• 

  WB analysis using 17673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 17673-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 17673-1-AP

  IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 17673-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17673-1-AP targets VPS4A/B in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11610

  Product name: Recombinant human VPS4B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-444 aa of BC039574

  Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

• Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 444 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC039574
• Gene Symbol: VPS4B
• Gene ID (NCBI): 9525
• RRID: AB_2215504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75351
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

Publications

What published studies show

The VPS4A/B Polyclonal antibody (Cat# 17673-1-AP) has 18 citations published in journals including Cell Research, Nature Cell Biology, Nature Communications, Science Advances, Developmental Cell, EMBO Journal, Journal of Cell Biology, Journal of Extracellular Vesicles, Nucleus, mBio, Viruses, Cancer Metabolism, Current Biology, iScience, Molecular Cell Oncology, Journal of Cell Science, and Current Issues in Molecular Biology. Research fields span exosome biogenesis, ESCRT machinery, autophagy, viral infection, endosomal trafficking, cancer metastasis, lipid droplet transfer, liver fibrosis, and neurodegeneration.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	J Extracell Vesicles; Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.; Authors - Wang Jiang
human	WB,IP,IF	Nat Commun; Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy; Authors - Takashi Nozawa ; KD Validated
human	WB	Nat Commun; Autophagic extracellular vesicles (AEVs) are distinct from exosomes and play crucial roles in viral infections.; Authors - Kedan Mao
mouse	WB	Sci Adv; Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.; Authors - Haiqiong Wang ; KD Validated