Validation Data Gallery
|Positive WB detected in||Y79 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, mouse skin tissue, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2400|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17673-1-AP targets VPS4A/B in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS4A/B fusion protein Ag11610|
|Full Name||vacuolar protein sorting 4 homolog B (S. cerevisiae)|
|Calculated molecular weight||444 aa, 49 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC039574|
|Gene ID (NCBI)||9525|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.
Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.
J Extracell Vesicles
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding.
J Cell Sci
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation
TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.
Nat Cell Biol
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.