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  • KD/KO Validated

XBP-1U specific Polyclonal antibody

XBP-1U specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25997-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1s, XBP-1S, XBP1U, XBP2



Tested Applications

Positive WB detected inrat spleen tissue, mouse spleen tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25997-1-AP is XBP-1U specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XBP-1U specific fusion protein Ag21714
Full Name X-box binding protein 1
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000938
Gene symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 25997-1-AP specially recognizes isoform XBP-1U.

Protocols

Product Specific Protocols
WB protocol for XBP-1U specific antibody 25997-1-APDownload protocol
IHC protocol for XBP-1U specific antibody 25997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

CNS Neurosci Ther

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Authors - Ming-Feng Zhou
humanWB

J Virol

Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.

Authors - Lei-Ke Zhang
  • KD Validated
ratWB,IF

J Ophthalmol

Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.

Authors - Shengyu Wu
mouseWB,IHC

Cell Biol Int

Human placenta-derived mesenchymal stem cells inhibit apoptosis of granulosa cells induced by IRE1α pathway in autoimmune POF mice.

Authors - Hongxing Li
mouseWB

Mol Ther Nucleic Acids

Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity.

Authors - Wenqin Xu
humanIHC,IF

Sci Adv

Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

Authors - Can Huang