CoraLite®555-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3D9A1

Cat no : CL555-66652

Synonyms

DKFZp781P0919, DNA repair protein XRCC2, XRCC2

---

Validation Data Gallery

• 

  FC experiment of HepG2 using CL555-66652

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human XRCC2 (CL555-66652, Clone:3D9A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66652 targets XRCC2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: XRCC2 fusion protein Ag14421
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 2
• Calculated Molecular Weight: 280 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC042137
• Gene Symbol: XRCC2
• Gene ID (NCBI): 7516
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.