CoraLite®594-conjugated XRCC2 Monoclonal antibody

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  IF Staining of L02 using CL594-66652

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated XRCC2 antibody (CL594-66652, Clone: 3D9A1 ) at dilution of 1:100, CL488-Phalloidin (green).

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  IF Staining of HepG2 using CL594-66652

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66652 (XRCC2 antibody) at dilution of 1:100.

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  FC experiment of HepG2 using CL594-66652

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC2 (CL594-66652, Clone:3D9A1) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

XRCC2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-66652

Clone No.3D9A1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1

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Tested Applications

• Positive IF/ICC detected in: L02 cells, HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66652 targets XRCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14421

  Product name: Recombinant human XRCC2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-280 aa of BC042137

  Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 2
• Calculated Molecular Weight: 280 aa, 32 kDa
• GenBank Accession Number: BC042137
• Gene Symbol: XRCC2
• Gene ID (NCBI): 7516
• RRID: AB_2883610
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43543
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.