Tested Applications
Positive WB detected in | HT-29 cells, HEK-293 cells, COLO 320 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IF | See 1 publications below |
Product Information
66852-1-Ig targets XRN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen |
CatNo: Ag27927 Product name: Recombinant human XRN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 651-950 aa of BC006417 Sequence: PFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVTYQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN Predict reactive species |
Full Name | 5'-3' exoribonuclease 2 |
Calculated Molecular Weight | 104 kDa |
Observed Molecular Weight | 109 kDa |
GenBank Accession Number | BC006417 |
Gene Symbol | XRN2 |
Gene ID (NCBI) | 22803 |
RRID | AB_2882192 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9H0D6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for XRN2 antibody 66852-1-Ig | Download protocol |
IHC protocol for XRN2 antibody 66852-1-Ig | Download protocol |
IF protocol for XRN2 antibody 66852-1-Ig | Download protocol |
FC protocol for XRN2 antibody 66852-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Cell Physiol Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p. | ||
Cancer Res NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer | ||
Cell Rep Transketolase attenuates the chemotherapy sensitivity of glioma cells by modulating R-loop formation |