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  • KD/KO Validated

YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 14392-1-AP

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse tissue
Positive IHC detected inmouse brain tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14392-1-AP targets YTHDC1 in WB, RIP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC1 fusion protein Ag5882
Full Name YTH domain containing 1
Calculated molecular weight 85 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC053863
Gene symbol YTHDC1
Gene ID (NCBI) 91746
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Protocols

Product Specific Protocols
WB protocol for YTHDC1 antibody 14392-1-APDownload protocol
IHC protocol for YTHDC1 antibody 14392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
humanWB

Carcinogenesis

YTH domain family 2 promotes lung cancer cell growth by facilitating 6-phosphogluconate dehydrogenase mRNA translation.

Authors - Hao Sheng
  • KD Validated
humanCoIP

Sci Rep

The m6A-methylase complex recruits TREX and regulates mRNA export.

Authors - Simon Lesbirel
mouseRIP

Dev Cell

N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

Authors - Jie Du
humanWB,IF

Nucleic Acids Res

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

Authors - Haojie Hao
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi