|Positive WB detected in||HEK-293 cells, MCF-7 cells, mouse tissue|
|Positive IHC detected in||mouse brain tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14392-1-AP targets YTHDC1 in WB, RIP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YTHDC1 fusion protein Ag5882|
|Full Name||YTH domain containing 1|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC053863|
|Gene ID (NCBI)||91746|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).
Med Sci Monit
Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.
YTH domain family 2 promotes lung cancer cell growth by facilitating 6-phosphogluconate dehydrogenase mRNA translation.
The m6A-methylase complex recruits TREX and regulates mRNA export.
N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.
Cell Death Differ
YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.
Nucleic Acids Res
N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.