Various lysates were subjected to SDS PAGE followed by western blot with 85023-2-RR (ZNF517 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85023-2-RR (ZNF517 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 85023-2-RR
Biolayer interferometry (BLl) kinetic assays of 85023-2-RR against Human ZNF517 were performed. The affinity constant is 0.855 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The ZNF family genes are an ancient group of mammalian paralogous homologs, and ZNF517 has been continuously lost during evolution, and although ZNF517 may be involved in transcriptional regulation, its exact function is unknown. A partial deletion and down-regulation of ZNF517 gene expression in cutaneous malignant melanoma (CMM) has been noted.
Various lysates were subjected to SDS PAGE followed by western blot with 85023-2-RR (ZNF517 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 85023-2-RR
Biolayer interferometry (BLl) kinetic assays of 85023-2-RR against Human ZNF517 were performed. The affinity constant is 0.855 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ZNF517 Recombinant antibody
Catalog Number
85023-2-RR
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
