Beta Actin Recombinant antibody
Beta Actin Recombinant Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, pig, rat, mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
150
CloneNo.
4H1
Cat no : 81115-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
Positive IHC detected in | rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells, A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:950-1:3800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 146 publications below |
IF | See 5 publications below |
Product Information
81115-1-RR targets Beta Actin in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
Tested Reactivity | Human, pig, rat, mouse |
Cited Reactivity | human, mouse, rat, chicken, pig |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Beta Actin fusion protein Ag14521 |
Full Name | actin, beta |
Calculated molecular weight | 375 aa, 42 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC002409 |
Gene symbol | ACTB |
Gene ID (NCBI) | 60 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
Protocols
Product Specific Protocols | |
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WB protocol for Beta Actin antibody 81115-1-RR | Download protocol |
IHC protocol for Beta Actin antibody 81115-1-RR | Download protocol |
IF protocol for Beta Actin antibody 81115-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Nano Long Noncoding RNA URB1-Antisense RNA 1 (AS1) Suppresses Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma by Driving Ferritin Phase Separation | ||
J Control Release Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility | ||
Cell Death Dis Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma | ||
Phytomedicine Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a | ||
Biomed Pharmacother Activation of Nrf2 inhibits ferroptosis and protects against oxaliplatin-induced ototoxicity | ||
Front Immunol LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma |