Beta Actin Recombinant antibody

Beta Actin Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, rat, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

150

CloneNo.

4H1

Cat no : 81115-1-RR

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Validation Data Gallery

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  WB analysis using 81115-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81115-1-RR (beta actin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 81115-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 81115-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 81115-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using beta actin antibody (81115-1-RR, Clone: 4H1 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue
• Positive IHC detected in: rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:950-1:3800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 146 publications below
• IF: See 5 publications below

Product Information

81115-1-RR targets Beta Actin in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Cited Reactivity: human, mouse, rat, chicken, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag14521
• Full Name: actin, beta
• Calculated molecular weight: 375 aa, 42 kDa
• Observed molecular weight: 42 kDa
• GenBank accession number: BC002409
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Publications

Species	Application	Title
		ACS Nano; Long Noncoding RNA URB1-Antisense RNA 1 (AS1) Suppresses Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma by Driving Ferritin Phase Separation; Authors - Yuan Gao
mouse	WB	J Control Release; Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility; Authors - Yabing Chen
human,mouse	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
	WB	Phytomedicine; Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a; Authors - Wenpeng Xie
human	WB	Biomed Pharmacother; Activation of Nrf2 inhibits ferroptosis and protects against oxaliplatin-induced ototoxicity; Authors - Kai Xu
human,mouse	WB	Front Immunol; LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma; Authors - Houhong Wang