Beta Actin Recombinant antibody

Beta Actin Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, rat, mouse and More (3)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4H1

Cat no : 81115-1-RR

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:950-1:3800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81115-1-RR targets Beta Actin in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Actin fusion protein Ag14521
Full Name actin, beta
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC002409
Gene Symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Protocols

Product Specific Protocols
WB protocol for Beta Actin antibody 81115-1-RRDownload protocol
IHC protocol for Beta Actin antibody 81115-1-RRDownload protocol
IF protocol for Beta Actin antibody 81115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Adv Mater

Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction

Authors - Dingyi Wang

ACS Nano

Long Noncoding RNA URB1-Antisense RNA 1 (AS1) Suppresses Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma by Driving Ferritin Phase Separation

Authors - Yuan Gao
mouseWB

Nat Commun

A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice

Authors - Yichao Lu

J Med Virol

Differential pathogenic and molecular features in neurological infection of SARS-CoV-2 Omicron BA.5.2 and BA.2.75 and Delta

Authors - Erlin Wang
humanWB

J Med Virol

SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species

Authors - Haiyun Yu
human,mouseWB

Research (Wash D C)

Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis

Authors - Lang Linghu