Beta Actin Recombinant antibody

Beta Actin Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, pig, rat, mouse and More (2)







Cat no : 81115-1-RR


ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, A549 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:950-1:3800
Sample-dependent, check data in validation data gallery

Product Information

81115-1-RR targets Beta Actin in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Actin fusion protein Ag14521
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )


Product Specific Protocols
WB protocol for Beta Actin antibody 81115-1-RRDownload protocol
IHC protocol for Beta Actin antibody 81115-1-RRDownload protocol
IF protocol for Beta Actin antibody 81115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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