Beta Actin Recombinant antibody, PBS Only

Beta Actin Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H1

Cat no : 81115-1-PBS

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

---

Validation Data Gallery

• 

  WB analysis using 81115-1-RR (same clone as 81115-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81115-1-RR (beta actin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 81115-1-RR (same clone as 81115-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using beta actin antibody (81115-1-RR, Clone: 4H1 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81115-1-PBS targets Beta Actin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag14521
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: ACTB
• Gene ID (NCBI): 60
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )