cIAP1 Monoclonal antibody

cIAP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1H3F1

Reviews (1)

Cat no : 66626-1-Ig

Synonyms

API1, BIRC2, C IAP1, cIAP1, Hiap 2, HIAP2, IAP 2, IAP homolog B, IAP2, MIHB, RING finger protein 48, RNF48

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Validation Data Gallery

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  WB analysis using 66626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66626-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66626-1-Ig (cIAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HepG2 cells, Hela cells, Jurkat cells, HSCT6 cells, pig brain tissue, rat brain tissue
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue, human lung cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

66626-1-Ig targets cIAP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: cIAP1 fusion protein Ag21398
• Full Name: baculoviral IAP repeat-containing 2
• Calculated Molecular Weight: 618 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC016174
• Gene Symbol: BIRC2
• Gene ID (NCBI): 329
• RRID: AB_2881986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

Publications

Species	Application	Title
human	WB	Cell Death Dis; Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.; Authors - Jingru Huangfu
human	WB	Chem Biol Interact; Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65; Authors - Jingwen Tan
human,mouse	WB,IHC,IF,IP	J Exp Clin Cancer Res; TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein; Authors - Xu Yang
mouse	WB,IF	JACC Basic Transl Sci; Bag3 Regulates Mitochondrial Function and the Inflammasome Through Canonical and Noncanonical Pathways in the Heart; Authors - JuFang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shubham (Verified Customer) (10-04-2019)

Good

• Applications: Immunoprecipitation,
• Cell Tissue Type: AC16

cIAP1 Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66626-1-Ig)