cIAP1 Monoclonal antibody

cIAP1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1H3F1

Cat no : 66626-1-Ig

Synonyms

API1, BIRC2, C IAP1, cIAP1, Hiap 2, HIAP2, IAP 2, IAP homolog B, IAP2, MIHB, RING finger protein 48, RNF48



Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue, pig brain tissue, HSCT6 cells, Jurkat cells, HepG2 cells, Hela cells, HEK-293 cells, A431 cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue, human lung cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66626-1-Ig targets cIAP1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen cIAP1 fusion protein Ag21398
Full Name baculoviral IAP repeat-containing 2
Calculated molecular weight 618 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016174
Gene symbol BIRC2
Gene ID (NCBI) 329
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

Protocols

Product Specific Protocols
WB protocol for cIAP1 antibody 66626-1-IgDownload protocol
IHC protocol for cIAP1 antibody 66626-1-IgDownload protocol
IF protocol for cIAP1 antibody 66626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.

Authors - Jingru Huangfu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shubham (Verified Customer) (10-04-2019)

Good

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: AC16
cIAP1 Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66626-1-Ig)