|Positive WB detected in||A431 cells, mouse brain tissue, rat brain tissue, pig brain tissue, HSCT6 cells, Jurkat cells, HepG2 cells, Hela cells, HEK-293 cells, A431 cells|
|Positive IHC detected in||human spleen tissue, human tonsillitis tissue, human lung cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66626-1-Ig targets cIAP1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||cIAP1 fusion protein Ag21398|
|Full Name||baculoviral IAP repeat-containing 2|
|Calculated molecular weight||618 aa, 70 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC016174|
|Gene ID (NCBI)||329|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).
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Shubham (Verified Customer) (10-04-2019)