CAR Detection Reagents
Reliable and scalable solutions for detection and quantification of Chimeric Antigen Receptor (CAR) engineered cells
Chimeric Antigen Receptor (CAR) based cell therapies are transforming treatments for cancer, autoimmune, and many other rare diseases. To support researchers developing and characterizing CAR engineered cells, Proteintech offers a comprehensive range of CAR detection reagents designed for high sensitivity, lot-to-lot consistency, and compatibility with various workflows.
Our reagents enable precise quantification and monitoring of CAR expression, ensuring robust and reproducible data in functional assays and clinical manufacturing settings. Each product is validated across multiple applications, offering flexibility for scientists working with diverse cell types and protocols. These reagents are well suited for Verification, In-Process Analytics of the manufacturing process, and Release Testing of the manufactured clinical product.
Recombinant manufacturing
Recombinant manufacturing ensures improved specificity, higher lot to lot consistency and reliable supply
Multi application specific
Compatible in flow cytometry, immunofluorescence, and IHC based applications for seamless integration into multiple workflows
Powered with FcZero technology
FcZero technology abolishes nonspecific FcγR interactions and eliminates the need for additional blocking steps in flow cytometry
Technology Overview and Validation
Universal CAR detection reagents
A tool designed to detect a broad range of CAR constructs through precise recognition of the scFv linker region
Our CAR-Linker antibodies are engineered to recognize the CAR scFv linker region, enabling precise and universal detection of a broad range of CAR constructs. Unlike conventional antigen-specific detection reagents, this design allows reliable identification and quantification of CAR expression regardless of target specificity.
Built on an FcZero-engineered antibody backbone, it minimizes non-specific Fc receptor interactions, producing cleaner data, lower background, and greater assay confidence. Incorporating AccuBright™ site-specific labeling technology, the reagent ensures uniform conjugation, enhanced brightness, and superior reproducibility across flow cytometry and related immunoassays.
Universal CAR detection mechanism of action
Flow Cytometry Validation of Universal CAR Detection Reagent
HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-GGGGS Linker Rabbit RecAb (CL647-FcA98262, Clone:242306C8). Cells were not fixed.
Immunofluorescence (IF) of Universal CAR Detection Reagent
Immunofluorescent analysis of CAR-Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunohistochemistry (IHC) Validation of Universal CAR Detection Reagent
Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens).
Target specific CAR detection
Flow Cytometry Validation of CD19 CAR Detection Reagent
HEK-293T cells or anti-CD19 (FMC63) CAR pool with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug FcZero-CAR™ Biotin-human CD19 CAR Detection Reagent (Biotin-CAR001) and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.
Target Specific CAR Detection Reagents
A tool engineered for precise and reliable target specific detection of CAR constructs in flow cytometry
The Target-Specific CAR Detection Reagent is purpose-built for flow cytometric detection and quantification of CAR-T cells. Featuring a native protein fused to a human IgG1 backbone, it enables direct, high-affinity binding to the target-binding region of CAR scFv, delivering clear and reproducible staining profiles that confirm both CAR expression and its interaction with the target.
Optimized for flow cytometry, the reagent provides precise discrimination between transduced and non-transduced cell populations, supporting both research and manufacturing quality control workflows. Consistent with the universal CAR-Linker reagent design, the target specific CAR Detection Reagent incorporates an FcZero-engineered backbone to minimize non-specific Fc receptor binding and utilizes AccuBright™ site-specific labeling for stable fluorescence and highly reproducible assay performance.
| Feature | Universal CAR detection reagent | Target-Specific CAR Detection Reagent |
|---|---|---|
| Detection Scope | Universal – detects CARs with linker across any target | Specific – detects only CARs binding a particular antigen (e.g., CD19) |
| Detection type | Antibody based detection targeting the linker | Antigen based detection targeting the antigen binding region of the CAR |
| Cost/Workflow Efficiency | Cost-effective – reduces need for multiple reagents, accelerates development | More expensive – each new CAR design increases cost and validation burden |
| Use Cases | Ideal for general CAR expression quantification, early process development, and cross-program analytics. | Best for program-specific functional studies, target-binding confirmation, and potency assays. |
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