luciferase Monoclonal antibody

luciferase Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

　 and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1F2C12

Cat no : 60158-1-Ig

Synonyms

dNGLUC, Luciferase

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60158-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60158-1-Ig (dNGLUC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

60158-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with 　 samples.

• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: luciferase fusion protein Ag13000
• Full Name: luciferase
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: AY015993
• RRID: AB_10665950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The immunogen of this antibody the luciferase(AY015993) from the copepod marine organism Gaussia princeps. This antibody is a McAb to Luciferase.

Publications

Species	Application	Title
mouse	WB	PLoS One; Multiplex Assay for Live-Cell Monitoring of Cellular Fates of Amyloid-β Precursor Protein (APP).; Authors - Maria Merezhko
human	WB	Mol Ther; Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization; Authors - Yuanjun Zhu