Gamma Catenin Polyclonal antibody

Gamma Catenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27872-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHT-29 cells, HUVEC cells, T-47D cells, mouse heart tissue, MCF-7 cells, rat heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27872-1-AP targets Gamma Catenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27394

Product name: Recombinant human JUP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 669-745 aa of BC000441

Sequence: TNSLFKHDPAAWEAAQSMIPINEPYGDDMDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA

Predict reactive species
Full Name junction plakoglobin
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC000441
Gene Symbol Gamma Catenin
Gene ID (NCBI) 3728
RRIDAB_2881000
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14923
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Gamma Catenin antibody 27872-1-APDownload protocol
IHC protocol for Gamma Catenin antibody 27872-1-APDownload protocol
IF protocol for Gamma Catenin antibody 27872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Oncol (Dordr)

The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.

Authors - Yizhi Xiao
humanWB,IF

BMC Cancer

Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines

Authors - Jueyao Zou
humanWB,IF

Int J Biol Macromol

Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation

Authors - Yining Liu
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