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Recombinant Human HVEM/TNFRSF14 protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 0.1-0.4 ng/mL

Cat no : Eg0015

Validation Data Gallery

  • Purity of Recombinant Human HVEM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human HVEM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human HVEM (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human LIGHT (His tag) with a linear range of 0.1-0.4 ng/mL.

    Immobilized Human HVEM (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human LIGHT (His tag) with a linear range of 0.1-0.4 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human HVEM (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human LIGHT (His tag) with a linear range of 0.1-0.4 ng/mL.
Expression HEK293-derived Human HVEM protein Leu39 -Val202 (Accession# Q92956-1) with a Myc tag and a His tag at the C-terminus.
GeneID 8764
Accession Q92956-1
PredictedSize 22.3 kDa
SDS-PAGE 28-38 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. The cytoplasmic region of this receptor was found to bind to several TNF receptor associated factor (TRAF) family members, which may mediate the signal transduction pathways that activate the immune response. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells.

References:

1.	Montgomery RI., et al. (1996) Cell. 1;87(3):427-36. 
2.	Kwon BS., et al. (1997) J Biol Chem. 30;272(22):14272-6. 
3.	Ware., et al. (2008). "Chapter 25: TNF-Related Cytokines in Immunity". In Paul, William (ed.). Fundamental Immunology (Book) (6th ed.)