Biotin-conjugated IgM Monoclonal antibody

human IgM Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

2D10B10

Cat no : Biotin-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, IGHM, MU, VH



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66484 targets human IgM in IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459
Full Name immunoglobulin heavy constant mu
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2918947
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

Protocols

Product Specific Protocols
IHC protocol for Biotin human IgM antibody Biotin-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols