CoraLite® Plus 647-conjugated IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2D10B10

Cat no : CL647-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, IGHM, MU, VH

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL647-66484

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 human IgM antibody (CL647-66484, Clone: 2D10B10 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66484 targets human IgM in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgM fusion protein Ag1459
• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_2920286
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).