• Featured Product
  • KD/KO Validated

CISD1 Polyclonal antibody

CISD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16006-1-AP

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, Mnt, ZCD1



Tested Applications

Positive WB detected inmouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue, rat skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16006-1-AP targets CISD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CISD1 fusion protein Ag8680
Full Name CDGSH iron sulfur domain 1
Calculated molecular weight 108 aa, 12 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC007043
Gene symbol CISD1
Gene ID (NCBI) 55847
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

Protocols

Product Specific Protocols
WB protocol for CISD1 antibody 16006-1-APDownload protocol
IHC protocol for CISD1 antibody 16006-1-APDownload protocol
IF protocol for CISD1 antibody 16006-1-APDownload protocol
IP protocol for CISD1 antibody 16006-1-APDownload protocol
FC protocol for CISD1 antibody 16006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway.

Authors - J-M Heo
humanWB

J Biol Chem

Parkin recruitment to impaired mitochondria for nonselective ubiquitylation is facilitated by MITOL.

Authors - Fumika Koyano
mouseWB

World J Gastrointest Pathophysiol

Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice.

Authors - Mandy Heritage
humanWB

Nat Struct Mol Biol

Mechanism and regulation of the Lys6-selective deubiquitinase USP30.

Authors - Malte Gersch
humanWB

Anticancer Res

Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae.

Authors - Andrew T Meram
ratWB

Lipids

Isolation of sarcolemmal plasma membranes by mechanically skinning rat skeletal muscle fibers for phospholipid analysis.

Authors - Fajardo Val Andrew VA