|Positive WB detected in||mouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue, rat skeletal muscle tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16006-1-AP targets CISD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CISD1 fusion protein Ag8680|
|Full Name||CDGSH iron sulfur domain 1|
|Calculated molecular weight||108 aa, 12 kDa|
|Observed molecular weight||14-17 kDa|
|GenBank accession number||BC007043|
|Gene ID (NCBI)||55847|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.
|Product Specific Protocols|
|WB protocol for CISD1 antibody 16006-1-AP||Download protocol|
|IHC protocol for CISD1 antibody 16006-1-AP||Download protocol|
|IF protocol for CISD1 antibody 16006-1-AP||Download protocol|
|IP protocol for CISD1 antibody 16006-1-AP||Download protocol|
|FC protocol for CISD1 antibody 16006-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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