Recombinant Mouse IL-1 alpha protein (His Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

His Tag

Activity

not tested

Cat no : Eg0263

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Validation Data Gallery

• 

  Purity of Mouse IL-1 alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse IL-1 alpha was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse IL-1 alpha protein Met+Ser115-Ser270 (Accession# P01582) with a His tag at the C-terminus.
• GeneID: 16175
• Accession: P01582
• PredictedSize: 21.9 kDa
• SDS-PAGE: 24-32 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.

References:

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4. Apte RN. et al. (2008). Immunol Rev. 222:222-41.
5. Palmer G. et al. (2005). Cytokine. 2:153-60.
6. Rider P. et al. (2013). Semin Immunol. 25(6):430-8.
7. Orjalo AV. et al. (2009). Proc Natl Acad Sci U S A. 106(40):17031-6.