p57Kip2 Monoclonal antibody

p57Kip2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12H3

Cat no : 66794-1-Ig

Synonyms

BWCR, BWS, CDKN1C, KIP2, p57, p57 Kip2, p57Kip2, WBS



Tested Applications

Positive WB detected inHSC-T6 cells, HeLa cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66794-1-Ig targets p57Kip2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p57Kip2 fusion protein Ag19927
Full Name cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Calculated molecular weight 316 aa, 32 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC067842
Gene symbol CDKN1C
Gene ID (NCBI) 1028
RRIDAB_2882138
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.

Protocols

Product Specific Protocols
WB protocol for p57Kip2 antibody 66794-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.

Authors - Giovanna Rigillo
mouseWB

Toxicology

Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice

Authors - Yong-Jiang Zhou