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NF-κB p65 Monoclonal antibody

NF-κB p65 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B12D11

Cat no : 66535-1-Ig

Synonyms

NF-kB, NFKB3, NF-κB, NF-κB p65, NF-κB3, p65, RELA, Transcription factor p65



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66535-1-Ig targets NF-κB p65 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Protocols

Product Specific Protocols
WB protocol for NF-κB p65 antibody 66535-1-IgDownload protocol
IHC protocol for NF-κB p65 antibody 66535-1-IgDownload protocol
IF protocol for NF-κB p65 antibody 66535-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Biochem Pharmacol

Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1.

Authors - Tu-Shuai Li
humanIF

Cancer Biol Med

Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis.

Authors - Caijun Zha
mouseWB

Biomed Res Int

Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4/NF-κB Signaling Pathway.

Authors - Wei Zhang
mouseIF

Respir Physiol Neurobiol

Xanthatin alleviates airway inflammation in asthmatic mice by regulating the STAT3/NF-κB signaling pathway.

Authors - Jingxia Chang
mouseWB

Biochem Biophys Res Commun

PMID: 31561855

Authors - Zhuo Wei
ratWB

Pflugers Arch

Interleukin-1β in hypothalamic paraventricular nucleus mediates excitatory renal reflex.

Authors - Fen Zheng