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NF-κB p65 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66535-1-Ig |CloneNo.: 1B12D11

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.


Images See all

NF-κB p65 Antibody WB HeLa cells 66535-1-Ig

Various cells were subjected to SDS PAGE followed by western blot with 66535-1-Ig (p65; RELA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB HEK-293 cells 66535-1-Ig

WB result of p65; RELA antibody (66535-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p65; RELA transfected HEK-293 cells

NF-κB p65 Antibody IHC human breast cancer tissue 66535-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig( p65; RELA antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-κB p65 Antibody IF HepG2 cells 66535-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 66535-1-Ig (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, rat, mouse
Positive WB detected in:
HeLa cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:3000
IHC : 1:150-1:600
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NF-κB p65 fusion protein Ag1199
Full name:
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight:
65 kDa
Observed molecular weight:
65 kDa
GenBank accession number:
BC011603
Gene ID (NCBI):
5970
Gene symbol
RELA
Synonyms
NFKB3, NF-κB3, p65, RELA, Transcription factor p65

Background

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.


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