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CL594-conjugated NF-κB p65 Antibody

NF-κB p65 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B12D11

Cat no : CL594-66535

Synonyms

NFKB3, p65, RELA, Transcription factor p65



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66535-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66535 targets CL594-conjugated NF-κB p65 in IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession number BC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated NF-κB p65 antibody CL594-66535Download protocol
Standard Protocols
Click here to view our Standard Protocols