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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFbb protein

Activity

Typically ≤ 3 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1308

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV


  • The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Animal-free Recombinant human PDGFbb (Catalouge: HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were plated and serum starved with 0.1% FBS for 24 hours and added increasing concentrations of recombinant human PDGFbb for 48 hours before addition of Prestoblue Cell Viability Reagent. EC50 is less than 5 ng/mL as determined by a 4 parameter non-linear regression model.

    Animal-free Recombinant human PDGFbb (Catalouge: HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were plated and serum starved with 0.1% FBS for 24 hours and added increasing concentrations of recombinant human PDGFbb for 48 hours before addition of Prestoblue Cell Viability Reagent. EC50 is less than 5 ng/mL as determined by a 4 parameter non-linear regression model.

"Recombinant PDGFbb Proteins" Comparison

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Technical Specifications

GeneID 5155
Species human
Expression HEK293
Activity Typically ≤ 3 ng/mL EC50
Purity >95%
Molecular Mass 29 to 32 kDa, homodimer, glycosylated
Formulation 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Publications

SpeciesTitle
human

Arch Biochem Biophys

Quiescin/sulfhydryl oxidase 1b (QSOX1b) induces migration and proliferation of vascular smooth muscle cells by distinct redox pathways.

Authors - Karime C França