- HEK293 expressed
- Animal-component free
HumanKine® recombinant human EGF protein- GMP grade
0.1-0.6 ng/mL EC50
3.04x10^6 IU/mg calibrated against EGF reference standard (NIBSC cat no: 91/530). Reference use only. Specific activity is not routinely tested
Cat no : HZ-1326-GMP
|EC50||0.1-0.6 ng/mL EC50|
|Specific Activity||3.04x10^6 IU/mg calibrated against EGF reference standard (NIBSC cat no: 91/530). Reference use only. Specific activity is not routinely tested|
|Molecular Mass||6 kDa, monomer, glycosylated|
|Formulation||1x PBS, See Certificate of Analysis for details|
Stability and Reconstitution
|Stability and Storage||Product Form||Temperature Conditions||Storage Time|
(From Date of Receipt)
|Lyophilized||-20°C to -80°C||Until Expiry Date|
|Lyophilized||Room Temperature||2 weeks|
|Reconstituted as per CofA||-20°C to -80°C||6 months|
|Reconstituted as per CofA||4°C||1 week|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution||Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).|
GMP Quality Policies
HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.
EGF (epidermal growth factor) is a 6 kDA, single chain protein that serves a mitogenic factor for numerous cell types, particularly epithelial cells and fibroblasts. It is predominantly produced and secreted by the kidneys in humans. EGF activates cells through binding with its corresponding receptor (EGFR). Receptor binding activates several downstream pathways leading to cell proliferation, differentiation, and apoptosis. EGF plays important roles in embryonic development, tissue regeneration, transport through ion channels, and wound healing. Dysregulated EGF signaling has been linked with renal disease progression, and EGF levels could potentially be used as biomarker to monitor disease-related cell damage (PMID: 15110798, 24513230, 27868030).