HumanKine® recombinant human EGF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.1-0.6 ng/mL EC50

Specific Activity

>1.00x10⁶ IU/mg

Species Reactivity

human,mouse,rat

Purity

> 95%

Publications

14

Reviews (1)

Cat no : HZ-1326

Synonyms

Urogastrone

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Validation Data Gallery

• 

  Purity of recombinant human EGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human EGF (HZ-1326) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant EGF for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-0.6 ng/mL.

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Technical Specifications

• GeneID: 1950
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.1-0.6 ng/mL EC50
• Specific Activity: >1.00x10^6 IU/mg
• Purity: > 95%
• Endotoxin: <1.0 EU/µg
• Accession Number: P01133
• Molecular Mass: 6 kDa reduced, 10 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

EGF (epidermal growth factor) is a 6 kDA, single chain protein that serves a mitogenic factor for numerous cell types, particularly epithelial cells and fibroblasts. It is predominantly produced and secreted by the kidneys in humans. EGF activates cells through binding with its corresponding receptor (EGFR). Receptor binding activates several downstream pathways leading to cell proliferation, differentiation, and apoptosis. EGF plays important roles in embryonic development, tissue regeneration, transport through ion channels, and wound healing. Dysregulated EGF signaling has been linked with renal disease progression, and EGF levels could potentially be used as biomarker to monitor disease-related cell damage (PMID: 15110798, 24513230, 27868030).

Synonyms

Urogastrone

Publications

Species	Title
mouse	Sci Adv; Varying mechanical forces drive sensory epithelium formation; Authors - Mingyu Xia
mouse	Nat Commun; Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells; Authors - Yang Sun
mouse	Am J Physiol Renal Physiol; A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition; Authors - Eoghainín Ó hAinmhire
human	Cancer Med; Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma; Authors - Zhen Fang
human	Med Sci Monit; Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study.; Authors - Shao-Chen Nie
mouse	Front Pharmacol; Bu-Gu-Sheng-Sui decoction promotes osteogenesis via activating the ERK/Smad signaling pathways; Authors - Ning Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K. (Verified Customer) (10-26-2023)

Used it in 100-500ng concentration for 10-30 minutes to check EGFR activation and always worked well.

• Applications: Western Blot,Immunofluorescence,Cell culture
• Primary Antibody Dilution: 200ng
• Cell Tissue Type: Human Liver cells