FcZero-rAb™ Biotin Anti-Human uPAR/CD87 Rabbit Recombinant Antibody

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  FC experiment of U-937 using Biotin-FcA98048

  1x10^6 PMA-treated U-937 cells were surface stained with 0.25 ug Biotin Anti-Human uPAR/CD87 Rabbit RecAb (Biotin-FcA98048, Clone: 240366G10) (red) or 0.25 ug FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated U-937 cells were surface stained with 0.25 ug Biotin Anti-Human uPAR/CD87 Rabbit RecAb (Biotin-FcA98048, Clone: 240366G10) (black, dashed). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

uPAR/CD87 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-FcA98048

Clone No.240366G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

PLAUR, 240366G10, CD87, MO3, U PAR

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Tested Applications

• Positive FC detected in: PMA treated U-937 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98048 targets uPAR/CD87 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: uPAR/CD87 fusion protein Eg0901
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_002659.4
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03405-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)