AP1B1 Polyclonal antibody

AP1B1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16932-1-AP

Synonyms

ADTB1, AP 1 complex subunit beta 1, AP105A, AP1B1, BAM22, Beta 1 adaptin, Beta adaptin 1, CLAPB2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

16932-1-AP targets AP1B1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1B1 fusion protein Ag10417
Full Name adaptor-related protein complex 1, beta 1 subunit
Calculated molecular weight 919 aa, 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC046242
Gene symbol AP1B1
Gene ID (NCBI) 162
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Protocols

Product Specific Protocols
WB protocol for AP1B1 antibody 16932-1-APDownload protocol
IF protocol for AP1B1 antibody 16932-1-APDownload protocol
IP protocol for AP1B1 antibody 16932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.

Authors - Arunas Kvainickas
humanWB

Am J Hum Genet

Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia.

Authors - Lynn M Boyden