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ASS1 Monoclonal antibody

ASS1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D1A11

Cat no : 66036-1-Ig

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1



Tested Applications

Positive WB detected inpig liver tissue, HeLa cells, human liver tissue, L02 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66036-1-Ig is ASS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9314
Full Name argininosuccinate synthetase 1
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC009243
Gene symbol ASS1
Gene ID (NCBI) 445
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
WB protocol for ASS1 antibody 66036-1-IgDownload protocol
IHC protocol for ASS1 antibody 66036-1-IgDownload protocol
IF protocol for ASS1 antibody 66036-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.

Authors - Qingyuan Feng
humanWB

BMC Cancer

PMID: 25240403

Authors - Jiangbo Liu
humanWB

FASEB J

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

Authors - Chung-Ying Tsai
  • KD Validated