CD40L/CD154 Polyclonal antibody

CD40L/CD154 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16668-1-AP

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP



Tested Applications

Positive WB detected inmouse small intestine tissue, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16668-1-AP targets CD40L/CD154 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD40L/CD154 fusion protein Ag10147
Full Name CD40 ligand
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC071754
Gene symbol CD40LG
Gene ID (NCBI) 959
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for CD40L/CD154 antibody 16668-1-APDownload protocol
IHC protocol for CD40L/CD154 antibody 16668-1-APDownload protocol
IF protocol for CD40L/CD154 antibody 16668-1-APDownload protocol
IP protocol for CD40L/CD154 antibody 16668-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bone

YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.

Authors - Liming Zhao
humanWB

Pharmacol Res

PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.

Authors - Giulia Bellini
mouseIHC

Br J Pharmacol

CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.

Authors - Fang Bian
mouseIHC

J Mol Cell Cardiol

TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ

Authors - Youzhi Zhang