- Featured Product
- KD/KO Validated
CD9 Monoclonal Antibody for IHC, WB, ELISA
Cat no : 60232-1-Ig
5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
|Positive WB detected in||A375 cells, rat liver tissue, rat spleen tissue, HeLa cells, A549 cells, L02 cells, HepG2 cells|
|Positive IHC detected in||human ovary tumor tissue, human colon cancer tissue, human breast cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
The 60232-1-Ig immunogen is Fusion Protein CD9 protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD9 fusion protein Ag14529|
|Full Name||CD9 molecule|
|Calculated molecular weight||228aa,25 kDa|
|Observed molecular weight||23-27 kDa|
|GenBank accession number||BC011988|
|Gene ID (NCBI)||928|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
Cell Death Dis
The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.
J Exp Clin Cancer Res
CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182.
Differential packaging of inflammatory cytokines/ chemokines and oxidative stress modulators in U937 and U1 macrophages-derived extracellular vesicles upon exposure to tobacco constituents.
Int J Mol Sci
Optimal Isolation Method of Small Extracellular Vesicles from Rat Plasma.
Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.
Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.