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CD9 Monoclonal antibody
CD9 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, PLA, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
4H7B9
Cat no : 60232-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, A549 cells, L02 cells, HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue, human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 32 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 60232-1-Ig is CD9 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD9 fusion protein Ag14529 |
| Full Name | CD9 molecule |
| Calculated molecular weight | 228 aa, 25 kDa |
| Observed molecular weight | 23-27 kDa |
| GenBank accession number | BC011988 |
| Gene symbol | CD9 |
| Gene ID (NCBI) | 928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD9 antibody 60232-1-Ig | Download protocol |
| IHC protocol for CD9 antibody 60232-1-Ig | Download protocol |
| IF protocol for CD9 antibody 60232-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis. | ||
J Exp Clin Cancer Res CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182. | ||
Cell Cycle Exosomes derived from autologous dermal fibroblasts promote diabetic cutaneous wound healing through the Akt/β-catenin pathway. | ||
Aging (Albany NY) Hypoxic pancreatic stellate cell-derived exosomal mirnas promote proliferation and invasion of pancreatic cancer through the PTEN/AKT pathway. | ||
Signal Transduct Target Ther The interferon-stimulated exosomal hACE2 potently inhibits SARS-CoV-2 replication through competitively blocking the virus entry. | ||
Aging (Albany NY) Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (03-22-2022) | Very weak bands with strong background were detected at 25 kDa
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