Validation Data Gallery
|Positive WB detected in
|A431 cells, HeLa cells
|Positive IHC detected in
|human ovary tumor tissue, human colon cancer tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human breast cancer tissue, human ovary tumor tissue, human lung cancer tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:1000-1:4000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60232-1-Ig targets CD9 in WB, PLA, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG1
|CD9 fusion protein Ag14529
|Calculated molecular weight
|228 aa, 25 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-22-2022)
Very weak bands with strong background were detected at 25 kDa