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CD9 Antibody

CD9 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4H7B9

Cat no : 60232-1-Ig

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29



Tested Applications

Positive WB detected inA375 cells, rat liver tissue, rat spleen tissue, HeLa cells, A549 cells, L02 cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue, human breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

The 60232-1-Ig immunogen is Fusion Protein CD9 protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD9 fusion protein Ag14529
Full Name CD9 molecule
Calculated molecular weight 228aa,25 kDa
Observed molecular weight 23-27 kDa
GenBank accession number BC011988
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 60232-1-IgDownload protocol
IHC protocol for CD9 antibody 60232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Authors - Zhenhong Ni
humanWB

J Exp Clin Cancer Res

CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182.

Authors - Zhi-Hong Zong
humanWB

PLoS One

Differential packaging of inflammatory cytokines/ chemokines and oxidative stress modulators in U937 and U1 macrophages-derived extracellular vesicles upon exposure to tobacco constituents.

Authors - Sanjana Haque
ratWB

Int J Mol Sci

Optimal Isolation Method of Small Extracellular Vesicles from Rat Plasma.

Authors - Kosuke Otani
humanWB

Oncogene

Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.

Authors - Jenna Marcus
mouseWB

Diabetes

Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.

Authors - Hui Zhao