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CD9 Antibody 1 Publications

Mouse Monoclonal| Catalog number: 60232-1-Ig |CloneNo.: 4H7B9

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

CD9 Antibody WB HeLa cells 60232-1-Ig

WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells.

CD9 Antibody WB A375 cells 60232-1-Ig

A375 cells, A549 cells, HeLa cells, L02 cells, and HepG2 cells were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

CD9 Antibody IHC human ovary tumor tissue 60232-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD9 Antibody IHC human ovary tumor tissue 60232-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, rat
Positive WB detected in:
A375 cells, rat liver tissue, rat spleen tissue, HeLa cells, A549 cells, L02 cells, HepG2 cells
Positive IHC detected in:
human ovary tumor tissue, human colon cancer tissue, human breast cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:1000-1:4000
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD9 fusion protein Ag14529
Full name:
CD9 molecule
Calculated molecular weight:
228aa,25 kDa
Observed molecular weight:
23-27 kDa
GenBank accession number:
BC011988
Gene ID (NCBI):
928
Gene symbol
CD9
Synonyms
5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

Background

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.


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