CD9 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD9 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B9

Cat no : 60232-1-PBS

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

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Validation Data Gallery

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  WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

  WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human CD9 (60232-1-Ig, Clone:4H7B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug isotype control (black). Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60232-1-PBS targets CD9 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD9 fusion protein Ag14529
• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.