- Featured Product
- KD/KO Validated
Biotin-conjugated CD9 Monoclonal antibody
CD9 Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IHC
Conjugate
Biotin
CloneNo.
4H7B9
Cat no : Biotin-60232
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-60232 targets CD9 in IHC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD9 fusion protein Ag14529 |
Full Name | CD9 molecule |
Calculated Molecular Weight | 228 aa, 25 kDa |
Observed Molecular Weight | 23-27 kDa |
GenBank Accession Number | BC011988 |
Gene Symbol | CD9 |
Gene ID (NCBI) | 928 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for Biotin CD9 antibody Biotin-60232 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |