CoraLite®594-conjugated CD9 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD9 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H7B9

Cat no : CL594-60232

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-60232

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL594-60232

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL594-60232

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-60232 is CD9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD9 fusion protein Ag14529
• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• RRID: AB_2919904
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.