CD98/SLC3A2 Monoclonal antibody

CD98/SLC3A2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B10F5

Cat no : 66883-1-Ig

Synonyms

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, SLC3A2



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, Raji cells, THP1 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66883-1-Ig targets CD98/SLC3A2 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD98/SLC3A2 fusion protein Ag7301
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight 68 kDa
Observed molecular weight 85-94 kDa, 120-130 kDa
GenBank accession numberBC001061
Gene symbol SLC3A2
Gene ID (NCBI) 6520
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
WB protocol for CD98/SLC3A2 antibody 66883-1-IgDownload protocol
IHC protocol for CD98/SLC3A2 antibody 66883-1-IgDownload protocol
FC protocol for CD98/SLC3A2 antibody 66883-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols