Alpha E-Catenin Monoclonal antibody

Alpha E-Catenin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7B8

Cat no : 66221-1-Ig

Synonyms

Alpha E catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66221-1-Ig targets Alpha E-Catenin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag23603
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC031262
Gene symbol CTNNA1
Gene ID (NCBI) 1495
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for Alpha E-Catenin antibody 66221-1-IgDownload protocol
IHC protocol for Alpha E-Catenin antibody 66221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.

Authors - Dan Sun