|Positive WB detected in||HeLa cells, pig brain tissue, MCF-7 cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, human skin cancer tissue, human liver cancer tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66379-1-Ig targets Beta Catenin in WB, IP, IHC, IF, COIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Full Name||catenin (cadherin-associated protein), beta 1, 88kDa|
|Calculated molecular weight||781 aa, 86 kDa|
|Observed molecular weight||92 kDa|
|GenBank accession number||NM_001904|
|Gene ID (NCBI)||1499|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols|
|WB protocol for Beta Catenin antibody 66379-1-Ig||Download protocol|
|IHC protocol for Beta Catenin antibody 66379-1-Ig||Download protocol|
|IF protocol for Beta Catenin antibody 66379-1-Ig||Download protocol|
|IP protocol for Beta Catenin antibody 66379-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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