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  • KD/KO Validated

Dynamin 1 Polyclonal antibody

Dynamin 1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18205-1-AP

Synonyms

DNM, DNM1, dynamin 1, Dynamin1



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, A549 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18205-1-AP targets Dynamin 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, chicken, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dynamin 1 fusion protein Ag12827
Full Name dynamin 1
Calculated Molecular Weight 851 aa, 96 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC063850
Gene Symbol DNM1
Gene ID (NCBI) 1759
RRIDAB_2093197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IHC protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IF protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IP protocol for Dynamin 1 antibody 18205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.

Authors - Xingyu Zhang
fishWB

Int J Mol Sci

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.

Authors - Shu-Wei Chen
humanWB

Commun Biol

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Authors - Christopher Noyes
  • KD Validated
humanWB

Neurobiol Dis

Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Authors - Li Zou
chickenWB

J Trace Elem Med Biol

Cu-induced mitochondrial dysfunction is mediated by abnormal mitochondrial fission through oxidative stress in primary chicken embryo hepatocytes.

Authors - Liuyan Wu