Filter: AllWBIPIHCIF/ICC

WB analysis using 24206-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24206-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 24206-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 24206-1-AP
IP result of anti-DNMT1 (IP:24206-1-AP, 4ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 1400 ug.
IHC staining of human lung cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 24206-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNMT1 antibody (24206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

DNMT1 Polyclonal antibody

Name: DNMT1 Polyclonal antibody
Brand: Proteintech
SKU: 24206-1-AP
Rating: 4.00 (2 reviews)

DNMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24206-1-AP

Publications(96)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Synonyms

AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1, DNMT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (96)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, C6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 85 publications below
IHC	See 5 publications below
IF	See 10 publications below
CoIP	See 2 publications below
ChIP	See 5 publications below
RIP	See 1 publications below

Product Information

24206-1-AP targets DNMT1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19116 Product name: Recombinant human DNMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1288-1632 aa of BC126227 Sequence: VSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD Predict reactive species
Full Name	DNA (cytosine-5-)-methyltransferase 1
Calculated Molecular Weight	1632 aa, 185 kDa
Observed Molecular Weight	180-200 kDa
GenBank Accession Number	BC126227
Gene Symbol	DNMT1
Gene ID (NCBI)	1786
RRID	AB_2879457
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26358
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

Protocols

Product Specific Protocols
IF protocol for DNMT1 antibody 24206-1-AP	Download protocol
IHC protocol for DNMT1 antibody 24206-1-AP	Download protocol
IP protocol for DNMT1 antibody 24206-1-AP	Download protocol
WB protocol for DNMT1 antibody 24206-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat,human	WB	Environ Int Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels Authors - Qiuju Liang View Article
human	WB	Cell Death Dis Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis Authors - Bowen Liu View Article
zebrafish	WB	Chemosphere Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae Authors - Fang Jiao View Article
human	WB,IF,ChIP	Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. Authors - Jin Wang KO Validated View Article
human	CoIP	Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. Authors - Lijie Zhou View Article
human	WB	J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy Authors - Liangliang Zhao View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Roy (Verified Customer) (09-17-2025)	Great band at the expected size. The dilution used was 1/1000 with an 1h incubation at Room Temperature Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa DNMT1 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:24206-1-AP)
FH Paula (Verified Customer) (12-14-2021)	good bands at the right molecular weight, but a lot of smear Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: fetal rat lung tissue DNMT1 Antibody Western Blot validation (1:500 dilution) in fetal rat lung tissue (Cat no:24206-1-AP)