Filter: AllWBIPIHCIF/ICC

WB analysis using 24206-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24206-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 24206-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 24206-1-AP
IP result of anti-DNMT1 (IP:24206-1-AP, 4ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 1400 ug.
IHC staining of human lung cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 24206-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 24206-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNMT1 antibody (24206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

DNMT1 Polyclonal antibody

Name: DNMT1 Polyclonal antibody
Brand: Proteintech
SKU: 24206-1-AP
Rating: 3.00 (1 reviews)

DNMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24206-1-AP

Publications(96)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Synonyms

AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1, DNMT

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (96)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, C6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 85 publications below
IHC	See 5 publications below
IF	See 10 publications below
CoIP	See 2 publications below
ChIP	See 5 publications below
RIP	See 1 publications below

Product Information

24206-1-AP targets DNMT1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNMT1 fusion protein Ag19116 Predict reactive species
Full Name	DNA (cytosine-5-)-methyltransferase 1
Calculated Molecular Weight	1632 aa, 185 kDa
Observed Molecular Weight	180-200 kDa
GenBank Accession Number	BC126227
Gene Symbol	DNMT1
Gene ID (NCBI)	1786
RRID	AB_2879457
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26358
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

Protocols

Product Specific Protocols
WB protocol for DNMT1 antibody 24206-1-AP	Download protocol
IHC protocol for DNMT1 antibody 24206-1-AP	Download protocol
IF protocol for DNMT1 antibody 24206-1-AP	Download protocol
IP protocol for DNMT1 antibody 24206-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat,human	WB	Environ Int Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels Authors - Qiuju Liang View Article
human	WB	Cell Death Dis Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis Authors - Bowen Liu View Article
zebrafish	WB	Chemosphere Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae Authors - Fang Jiao View Article
human	WB,IF,ChIP	Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. Authors - Jin Wang KO Validated View Article
human	CoIP	Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. Authors - Lijie Zhou View Article
human	WB	J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy Authors - Liangliang Zhao View Article