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DNMT1 Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 24206-1-AP

Featured Product KD/KO validated

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Con: 61 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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DNMT1 Antibody IP HEK-293 cells 24206-1-AP

IP Result of anti-DNMT1 (IP:24206-1-AP, 5ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 2500ug.

DNMT1 Antibody WB HEK-293 cells 24206-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP( DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

DNMT1 Antibody IF HepG2 cells 24206-1-AP

Immunofluorescent analysis of HepG2 cells using 24206-1-AP(DNMT1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, rat, Chicken, gilt, hen, pig
Positive WB detected in:
HEK-293 cells, C6 cells
Positive IP detected in:
HEK-293 cells
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DNMT1 fusion protein Ag19116
Full name:
DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight:
1632aa,185 kDa
Observed molecular weight:
180-200 kDa
GenBank accession number:
BC126227
Gene ID (NCBI):
1786
Gene symbol
DNMT1
Synonyms
AIM, CXXC9, DNA MTase HsaI, DNMT, DNMT1, MCMT

Background

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MV 184 kDa and 145 kDa. (PMID: 10753866)


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