E-cadherin Antibody 102 Publications

Rabbit Polyclonal| Catalog number: 20874-1-AP

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Freight/Packing

Con: 51 μg/150 μl

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Tested Applications

Applications:
ELISA, FC, IF, IHC, IP, WB
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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse testis tissue,A431 cells, DU 145 cells, MCF-7 cells

Positive IP detected in:
A431 cells

Positive IHC detected in:
human breast cancer tissue,human hepatocirrhosis tissue, human skin tissue, mouse liver tissue, human colon tissue

Positive IF detected in:
A431 cells,human breast cancer tissue

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cadherin 1, type 1, E-cadherin (epithelial)

Calculated molecular weight:
882aa,97 kDa

Observed molecular weight:
125 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CDH1

Synonyms
Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, LCAM, UVO, Uvomorulin
Background

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-kDa intact E-cadherin and its 80-kDa trypsin-cleaved fragment.


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