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  • KD/KO Validated

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20874-1-AP

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin



Tested Applications

Positive WB detected inT-47D cells, DU 145 cells, A431 cells, mouse testis tissue, MCF-7 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue, human hepatocirrhosis tissue, mouse liver tissue, human colon tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, human breast cancer tissue, mouse embryo tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20874-1-AP is E-cadherin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, cow, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-kDa intact E-cadherin and its 80-kDa trypsin-cleaved fragment.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
FC protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
humanWB,IF

Front Physiol

Keratinocytes Isolated From Individual Cleft Lip/Palate Patients Display Variations in Their Differentiation Potential in vitro.

Authors - Martin Degen
humanWB

Med Sci Monit

PMID: 30605443

Authors - Lin Chen
humanWB,IHC

Theranostics

miR-200c/141 Regulates Breast Cancer Stem Cell Heterogeneity via Targeting HIPK1/β-Catenin Axis.

Authors - Bingjie Liu
mouseWB

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
humanWB,IF

Mol Med Rep

PMID: 30628686

Authors - Ying Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Martin (Verified Customer) (12-13-2017)

  • Applications: Western Blot, Immunofluorescence