GFAP Antibody

GFAP Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B2E10

Cat no : 60190-1-Ig

Synonyms

GFAP



Tested Applications

Positive WB detected inHuman brain, human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue, human cerebellum tissue, U-251 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, mouse brain tissue, rat brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:10000
Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Product Information

60190-1-Ig targets GFAP in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Reactivity human, mouse, rat, pig
Cited Species human, mouse, rabbit, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFAP fusion protein Ag10452
Full Name glial fibrillary acidic protein
Calculated molecular weight 432aa,50 kDa
Observed molecular weight 45-52 kDa
GenBank accession number BC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Function
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer’s and Parkinson’s disease), and neurotrauma.
Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.



Protocols

Product Specific Protocols
WB protocol for GFAP antibody 60190-1-IgDownload protocol
IHC protocol for GFAP antibody 60190-1-IgDownload protocol
IF protocol for GFAP antibody 60190-1-IgDownload protocol
IP protocol for GFAP antibody 60190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Toxicol Sci

Targeting Smox is neuroprotective and ameliorates brain inflammation in cerebral ischemia/reperfusion rats.

Authors - Jiawei Fan
IF

Neurochem Res

Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.

Authors - Liu Xiaoying
mouseIF

Eur J Neurosci

Dynamic Cell Type-specific Expression of Nrf2 After Traumatic Brain Injury in Mice.

Authors - Wenwen Dong
ratIF

Int J Biochem Cell Biol

Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor.

Authors - Xuming Yu
ratIF

J Neurosurg

Annexin A7 induction of neuronal apoptosis via effect on glutamate release in a rat model of subarachnoid hemorrhage.

Authors - Qing-Song Lin

Front Mol Neurosci

L-Cysteine-Derived H2S Promotes Microglia M2 Polarization via Activation of the AMPK Pathway in Hypoxia-Ischemic Neonatal Mice.

Authors - Xin Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mai Khatri (01-30-2020)

The antibody seemed to have some background. I will repeat the experiment with different dilutions and conditions to find an optimum dilution for this antibody.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Brain Tissue
No image submitted with review
FH

George Wardley (08-27-2019)

This gave good staining for activated astrocytes in the 488 channel (1:500 secondary dilution) however there was also significant endogenous background staining which made analysis difficult.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Spinal Cord Mouse Slice Culture
No image submitted with review
FH

Chintan Koyani (06-03-2019)

This antibody was used to detect Glial fibrillary acidic protein in primary mouse microglia cells using Western blot. Antibody worked quite well and gave a very specific band near 50 kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: Mouse microglia
GFAP Antibody Western Blot, validation (1:10,000 dilution) in Mouse microglia (Cat no:60190-1-Ig)
FH

Di Hu (12-18-2018)

Good antibody!

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: Neurons
No image submitted with review
FH

Lalitha Kurada (12-13-2018)

Reliable results.

  • Applications: Western Blot,Immunohistochemistry,
  • Cell Tissue Type: Mice Brain
No image submitted with review
FH

Chandrakanth Edamakanti (12-06-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cerebellum
No image submitted with review
FH

Emily Plyler (11-29-2018)

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse brain sections, mouse whole retina, mouse optic nerve sections
No image submitted with review