Human APOB ELISA Kit

Sensitivity

0.68 ng/mL

Range

3.9-250 ng/mL

Reactivity

Human

Cat no : KE00158

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB



Product Information

KE00158 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOB ELISA kit is to be used to detect and quantify protein levels of endogenous APOB. The assay recognizes human APOB. An antibody specific for APOB has been pre-coated onto the microwells. The APOB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human APOB is added to detect the captured human APOB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human APOB ELISA Kit
Tests 1 X 96 well plate
Sample type Serum,Plasma, Cell Culture supernatants, Human milk
Assay type Sandwich
Sensitivity 0.68 ng/mL
Range 3.9-250 ng/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human Serum95%77%-121%
Cell Culture supernatants103%86%-122%
Human milk102%75%-122%

IntraAssay

Samplenmean ( ng/mL)SDCV%
120119.94.13.4
22028.30.72.6
3207.20.45.4

InterAssay

Samplenmean ( ng/mL)SDCV%
124133.39.87.3
22431.02.47.9
3248.00.810.0

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB gene encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. APOB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. APOB-48 and APOB-100 share a common N-terminal sequence, but APOB-48 lacks APOB-100's C-terminal LDL receptor binding region. Plasma APOB levels are associated with coronary heart disease.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB