Cytokeratin 20 Antibody
Cytokeratin 20 Monoclonal Antibody for IHC, WB, ELISA
Cat no : 60183-1-Ig
CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT
|Positive WB detected in||Colo320 cells, COLO 320 cells|
|Positive IHC detected in||human colon tissue, human stomach tissue, human urothelial carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:51200|
|Sample-dependent, check data in validation data gallery|
60183-1-Ig targets Cytokeratin 20 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Cytokeratin 20 fusion protein Ag11336|
|Full Name||keratin 20|
|Calculated molecular weight||424aa,48 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC031559|
|Gene ID (NCBI)||54474|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
Biochem Biophys Res Commun
Construction of artificial human peritoneal tissue by cell-accumulation technique and its application for visualizing morphological dynamics of cancer peritoneal metastasis.
Cell Mol Gastroenterol Hepatol
Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer.